Lotman's “structure-semiotics” refers both to the structure research and semiotic study.With a scientific property and rich cultural connotation, it vividly interprets the approaches and methods of humanism.The polyphony and humanism in Lotman's semiotics not only save the modernism'smethods and its rational power, but also the postmodernism's methods and its lulti position.Therefore lotman's semiotics shows the compatibility and the harmonious coexistence, which has important reference value and theoretical significance to the cultural and literary studies in teh post theoretical era.