Abstract: The ideology of people’s subjectivity is the core thread running through Marxist theory and the fundamental starting point for the development of socialism with Chinese characteristics in the new era, possessing rich theoretical connotations. Based on a comprehensive study and interpretation of the connotation of Marxist people’s subjectivity, this article analyzes its theoretical characteristics and systematically grasps the transcendence and breakthrough of Marx’s people’s subjectivity theory from three dimensions: history, reality, and future. At the same time, it deeply summarizes its practical approach and creative application from three aspects: theoretically arming the masses, implementing the mass line, and improving the whole-process of people’s democratic operation mechanism, laying the foundation for inspiring the subjective consciousness of people to start entrepreneurship and promoting Chinese-style modernization.