Abstract: Xi Jinping Thought on Culture has structured system and rich connotations, and its integration into the “Great Ideological and Political Courses” has strong contemporary value and social significance. Deeply understanding the value and implications of integrating Xi Jinping Thought on Culture into the “Great Ideological and Political Courses” in universities and actively exploring practical paths is a timely action for universities to shoulder the new cultural mission of the new era. It is a necessary action to cultivate and practice the core socialist values. It is a practical need to enhance the cohesion and leadership of socialist ideology. It is a targeted strategy to enhance the effectiveness of “Great Ideological and Political Courses” in cultivating students’ souls. In terms of practical path, it is necessary to integrate into teaching classroom by putting effort into content design and teaching methods; to integrate into the practice classroom by putting effort into making good use of the social classroom; to integrate into the teaching staff, efforts should be made in scientific research and building a strong team; to integrate into the network, efforts should be made to deeply cover the ‘thought cloud’.