Abstract: The report of the 20th National Congress of the Communist Party of China points out the direction and provides the guidance for strengthening the construction of grassroots Party organizations in colleges and universities. Promoting and strengthening the brand cultivation of grass-roots party organizations in colleges and universities is a practical path to build a high-quality party building system. It plays a promoting role in helping colleges and universities to build a powerful country in education and implement the fundamental task of cultivating morality and people. Also, it is conducive to demonstrating and driving all grassroots party organizations to make comprehensive progress. The practical experience and logic contained in the third batch of party building demonstration and quality excellence cultivation results in colleges and universities have clarified the construction direction and core points of the cultivation and creation of the brand cultivation of grassroots party organizations in colleges and universities. The brand cultivation of grassroots party organizations in colleges and universities should follow the theoretical requirements of party construction and the discipline concept of brand science. It should take brand positioning, brand design, brand cultivation and brand promotion as the practice path of cultivating and establishing. Therefore, the theory, demand and functional elements of brand cultivation of grassroots party organizations in colleges and universities should run through every link and whole process of brand cultivation and establishment.