Abstract: With Beijing Institute of Iron and Steel Technology( 1952-1966) as an example, which was founded in the adjustment period in 1950 s, the paper briefly expounds the connotation of discipline culture, analyses the influence of discipline culture on the formation of university culture from the aspects of sculpture and architecture, art and poetry, concept and ethos, compares the cultural differences and conflicts between the disciplines, and provides a reference for the understanding of disciplinary factors in the culture formation of the new colleges and universities in the period.